ORIGINAL ARTICLE

JOP. J Pancreas (Online) 2006; 7(5):454-464.

Pro-Inflammatory Cytokines Affect Pancreatic Carcinoma Cell. Endothelial Cell Interactions

Miranda ten Kate1, Leo J Hofland2, Peter M van Koetsveld2, Johannes Jeekel1, Casper HJ van Eijck1

1Department of Surgery and 2Department of Internal Medicine, Erasmus Medical Center. Rotterdam, the Netherlands

SOURCE DATA

Load the file with data of Figure 1: 05_data01.xls

Variable name

Description

Coded values/Units

ID

Sample identifier

 

GR

Group

0 : Control
1 : IL-1beta (0.1 ng/mL)
2 : IL-1beta (10 ng/mL)
3 : TNF (0.1 ng/mL)
4 : TNF (10 ng/mL)
5 : IL-6 (0.1 ng/mL)
6 : IL-6 (10 ng/mL)

T

Pre-incubation time

1 : 4 h
2 : 12 h

PANC

Adhesion of PanC1 cells

% vs. control

MIAPACA

Adhesion of MiaPaCa cells

% vs. control

BXPC3

Adhesion of BxPC3 cells

% vs. control

Load the file with data of Figure 2: 05_data02.xls

Variable name

Description

Coded values/Units

ID

Sample identifier

 

GR

Group

0 : Control
1 : IL-1beta (0.1 ng/mL)
2 : IL-1beta (10 ng/mL)
3 : TNF (0.1 ng/mL)
4 : TNF (10 ng/mL)
5 : IL-6 (0.1 ng/mL)
6 : IL-6 (10 ng/mL)

ESEL

Anti-E-selectin expression

OD

ICAM

Anti-ICAM-1 expression

OD

VCAM

Anti-VCAM-1 expression

OD

Load the file with data of Figure 3: 05_data03.xls

Variable name

Description

Coded values/Units

ID

Sample identifier

 

GR

Group

1 : Untreated
2 : IL-1beta
3 : Anti-E-selectin, untreated
4 : Anti-E-selectin, IL-1beta
5 : Anti-ICAM-1, untreated
6 : Anti-ICAM-1, IL-1beta
7 : Anti-E-selectin + Anti-ICAM-1, IL-1beta
8 : Anti-VCAM-1, untreated
8 : Anti-VCAM-1, IL-1beta

PANC

Adhesion of PanC1 cells

% vs. control

MIAPACA

Adhesion of MiaPaCa cells

% vs. control

BXPC3

Adhesion of BxPC3 cells

% vs. control

Load the file with data of Figure 4: 05_data04.xls

Variable name

Description

Coded values/Units

ID

Sample identifier

 

GR

Group

1 : E-selectin
2 : ICAM-1
3 : VCAM-1
4 : sLea
5 : sLex
6 : LFA-1
7 : VLA-1

IL1b

Pre-incubation

0 : Basal (untreated)
1 : IL-1beta

PANC

Expression on PanC1 cells

OD

MIAPACA

Expression on MiaPaCa cells

OD

BXPC3

Expression on BxPC3 cells

OD

Load the file with data of Figure 5: 05_data05.xls

Variable name

Description

Coded values/Units

ID

Sample identifier

 

IL1B

Pre-incubation with IL-1beta

0 : No (control MEC)
1 : Yes (MEC + IL-1beta)

BXPC3

Pre-incubation with BxPC3

0 : No (control BxPC3)
1 : Yes (Pre-incubated BxPC3)

ADHE

Expression

OD

STATISTICAL ANALYSIS

Load the statistical report: 05_stat.html


Abstract
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Correspondence Casper HJ van Eijck: c.vaneijck@erasmusmc.nl


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