International Symposium on "HCO3- AND CYSTIC FIBROSIS". San Diego, CA (USA). March 3-5, 2001.
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Sodium-Coupled Bicarbonate Transporters
Walter F Boron
Department of Cell and Molecular Physiology, Yale University School of Medicine. New Haven, CT, USA
Together, the Na+-coupled HCO3- transporters and the AE family of anion exchangers (i.e., Cl-HCO3 exchangers) comprise the bicarbonate transporter (BT) superfamily. Virtually all BTs are important for the regulation of intracellular pH (pHi) in cells throughout the body. Specific BTs also play roles in cell-volume regulation, as well as for the transport of salt and/or acid-base equivalents across many epithelia. Electrogenic Na/HCO3 cotransporters (NBCe’s) play key roles in HCO3- reabsorption by the renal proximal tubule, and HCO3- secretion by the pancreatic duct. Electroneutral NBC’s (NBCn’s) regulate pHi in vascular smooth muscle and are present in/near axons in the brain. Finally, the Na+-driven Cl-HCO3 exchanger (NDCBE’s) appear to be the major pHi regulators in CNS neurons. A characteristic of most, but not all, BT’s is that they are inhibited rather effectively by 4,4'-diisothiocyanostilbene-4,4'-disulfonate (DIDS).
Anion Exchangers (AEs)
The founding BT-superfamily member is the Cl-HCO3 exchanger (Figure 1A), described decades ago in RBCs, and cloned 16 years ago by Kopito and Lodish  as the anion exchanger AE1. In other cells, Cl-HCO3 exchangers (AE2, AE3) normally functions as acid loaders. Recently, AE4 (more closely related to the NBCs than the AEs) was cloned from kidney, and may represent the apical Cl-HCO3 exchanger in beta-intercalated cells . AE1 has long been thought to exist as a dimer, a view supported by cryo-EM studies to a 20-angstrom resolution .
Figure 1. HCO3- transporters
Na+-Driven Cl--HCO3- Exchanger (NDCBE)
The Na+-driven Cl-HCO3 exchanger (Figure 1B) was first described by Roger Thomas and by De Weer, Russell and Boron, working on snail neurons [4, 5, 6], squid axons [7, 8, 9] and barnacle muscle . This was the first transporter shown to be involved in pHi regulation. We have now cloned the Na+-driven Cl-HCO3 exchanger from human brain (NDCBE1) and also from squid neurons (SF1). Mike Romero cloned from Drosophila a related cDNA (NDAE) that appears to encode a Na+-driven Cl-anion (i.e., OH–) exchanger . Wang et al. has cloned a related mouse cDNA, not yet well characterized physiologically .
NDCBE1 appears to be the major pHi regulator in many neurons. It is highly expressed in brain and testis . At the amino-acid level, NDCBE1 is about 50% identical to NBCe1 and about 75% identical to NBCn1. NDCBE requires Na+, HCO3- and Cl–. It is electroneutral, not associated with any channel activity (see NBCn1 below), and is highly sensitive to DIDS. 36Cl-flux measurements show that the unidirectional Cl– efflux requires external Na+ and HCO3-, and blocked by DIDS. Using Na+ electrodes to measure the DIDS-sensitive net Na+ efflux, we found that the HCO3- to Na+ stoichiometry is 2:1, as expected for a Na+-driven Cl-HCO3 exchanger.
Electrogenic Na/HCO3 Cotransporter (NBCe) with 1:3 Stoichiometry
The electrogenic Na/HCO3 cotransporter was first described by Boulpaep and Boron in the salamander renal proximal tubule . This transporter mediates the basolateral step of HCO3- reabsorption in the proximal tubule. Because this transporter has a Na+:HCO3- stoichiometry of 1:3 (Figure 1C), it mediates net HCO3- efflux at the resting membrane voltage (Vm). We expression cloned this transporter from the salamander kidney (Figure 2: NBCe1-A), the first of the cation-coupled HCO3-transporters to be cloned . Soleimani and coworkers later cloned it from human kidney , and we, from rat kidney .
An amino-acid sequence alignment of the various AEs led to a proposed consensus motif at which DIDS covalently reacts with the AEs: KLXK (X=I,Y) , where the first K is K539. The predicted location of this site is at the extracellular face of the fifth membrane-spanning segment. At the homologous site, NBCe1 has KMIK, suggesting a motif of KZXK (Z=M,L).
Electrogenic Na/HCO3 Cotransporter (NBCe) with 1:2 Stoichiometry
In 1989, Deitmer and Schlue described an inwardly directed electrogenic Na/HCO3 cotransporter in leech glial cells . Based on known values of [Na+]i, pHi and Vm, they concluded that the stoichiometry must be 1:2. Others subsequently described inwardly directed electrogenic Na/HCO3 cotransporters in several mammalian cells, including pancreatic duct cells [20, 21]; we functionally identified it in rat astrocytes . Kurtz , as well as our group , cloned the "pancreatic" NBCe (Figure 2: NBCe1-B), which is a splice variant of NBCe1-A. We also cloned a "brain" NBCe (Figure 2: NBCe1-C) , and showed that an NBCe protein is present in astrocytes  and the basolateral membrane of pancreatic duct . NBCe1-B plays a critical role in HCO3- secretion by the pancreatic duct.
Figure 2. Alternative splicing of NBCe
A key issue is what determines whether NBCe1 operates with a 1:3 or 1:2 stoichiometry? Is it a difference in amino-acid sequence, posttranslational modification, formation of a heterodimer with an as-yet unidentified NBC, or an additional subunit? Alternative splicing (Figure 2) makes the N terminus of renal NBCe (NBCe1-A, which mediates HCO3- efflux in situ) quite different from that of either pancreatic or brain NBCe (NBCe1-B and -C, which mediate HCO3-influx in situ).
The ionic mechanism of NBCe1 has not been investigated. Figure 3 summarizes potential mechanisms. If the same protein (i.e., one of the NBCe1 isoforms) must function with two different stoichiometries, then the simplest way to accomplish this feat would be to shift from binding HCO3- to binding CO32- at one site (Figure 3: A® E). Other transitions would require introducing a new binding site (Figure 3: A® D, B® E, C® F).
Figure 3. Models of electrogenic Na+/HCO3- cotransport
Electroneutral Na/HCO3 Cotransporter (NBCn)
In the late 1980s and early 1990s, several groups described an apparently electroneutral Na/HCO3 cotransporter (1:1 stoichiometry) that extrudes acid from oligodendrocytes , vascular smooth muscle , cardiac Purkinje fibers  and cardiac myocytes . Because of problems depleting cells of Cl– or measuring very small electrical changes, it was not entirely clear that an electroneutral Na/HCO3 cotransporter even existed. We cloned NBCn1 ("n"=neutral) from rat aorta  and demonstrated, in oocytes, that it is indeed electroneutral and independent of Cl–.
As a HCO3- cotransporter, NBCn1 is only very weakly inhibited by DIDS. This observation is not surprising, inasmuch as the consensus DIDS-reaction motif is disrupted in NBCn1. At the position homologous to the KLXK in AE1, NBCn1 has KLFH. We hypothesized that we could increase NBCn1’s DIDS sensitivity by serially converting NBCn1’s KLFH to KLXK. Our first NBCn1 mutant (H751K, which yields KLFK) is 100% blocked by 500 m M DIDS. Thus, our data suggests that a more general DIDS-reaction consensus sequence is KZYK (Z=L,M,T,V, and X=F,I,M,T,V,Y).
A major surprise was that, even though NBCn1 is electroneutral in its transport mode, expression of NBCn1 is associated with a Na+-channel activity. This Na+ current is that unlike any described elsewhere, and that is stimulated by DIDS. The NBCn1-associated Na+-channel property could represent a channel native Xenopus oocyte (e.g., its expression could be triggered by expressing NBCn1). Alternatively, the Na+ channel could represent "slippage" of NBCn1, a view supported by the DIDS result. We have attempted to eliminate this Na+-channel property by generating chimeras between NBCe1 and NBCn1. Replacing the putative cytoplasmic N or C termini of NBCn1 has no effect on either the electroneutrality of NBCn1 in its transport mode, nor on the Na+-channel property of NBCn1.
Our rat NBCn1 clone is closely related (about 90% identical at the amino-acid level), though not identical to a human clone described by Pushkin et al. , and that maps to chromosome 3 . Because they did not measure Vm or current, Pushkin et al. could not come to any firm conclusions about electrogenicity/neutrality. Curiously, their clone (unlike ours) in oocytes is inhibited by ethylisopropylamiloride (EIPA, an inhibitor of Na-H exchange) and functions without HCO3-. Preliminary data from Kurtz suggests that the potential PDZ-binding domain at the C terminus of NBCn1 interacts with the vacuolar ATPase .
Cl-HCO3 exchangers are present in many mammalian cells, and were long thought to be the sole acid loader for non-epithelial cells. However, we found that the pHi recovery (i.e., a decrease in pHi) from alkaline loads by rat neurons or astrocytes is neither Cl– dependent nor DIDS sensitive. The molecular substrate of this pHi recovery may be the K/HCO3 cotransporter, in squid axons [35, 36, 37]. K/HCO3 cotransporter is not blocked by DIDS, but by quaternary amines. Preliminary data show that phenyl-propyltetraethylammonium (PPTEA+) blocks recovery from alkaline loads in astrocytes.
Other HCO3- Transporters
Perhaps related, very distantly to the BT superfamily is the sulfate anion transporter (SAT) family. SAT, which can exchange sulfate for CO32-, is present at the basolateral membranes of small intestine and renal proximal tubule. Another SAT-family member, DRA (down-regulated in adenoma) encodes a Cl-HCO3 exchanger present in apical membranes of certain small-intestine cells  and pancreatic ducts . Along with cystic fibrosis transmembrane conductance regulator (CFTR) and NBCe1-B, DRA may play a critical role in the secretion of HCO3- by the pancreatic duct. Pendrin, which is related to DRA and functions as a Cl-formate exchanger , may also transport HCO3- .
Kopito RR, Lodish HF. Primary structure and transmembrane orientation of the murine anion exchange protein. Nature 1985; 316:234-8. [More details]
Tsuganezawa H, Kobayashi K, Iyori M, Araki T, Koizumi A, Watanabe SI, et al. A new member of the HCO3- transporter superfamily is an apical anion exchanger of beta-intercalated cells in the kidney. J Biol Chem 2001; 276:8180-9 [More details]
Wang DN, Sarabia VE, Reithmeier RA, Kuhlbrandt W. Three-dimensional map of the dimeric membrane domain of the human erythrocyte anion exchanger, Band 3. EMBO J 1994; 13:3230-5. [More details]
Thomas RC. The effect of carbon dioxide on the intracellular pH and buffering power of snail neurones. J Physiol 1976; 255:715-35. [More details]
Thomas RC. Ionic mechanism of the H+ pump in a snail neurone. Nature 1976; 262:54-5. [More details]
Thomas R C. The role of bicarbonate, chloride and sodium ions in the regulation of intracellular pH in snail neurones. J Physiol 1977; 273:317-38. [More details]
Boron WF, De Weer P. Active proton transport stimulated by CO2/HCO3- blocked by cyanide. Nature 1976; 259:24O-1. [More details]
Russell JM, Boron WF. Role of chloride transport in regulation of intracellular pH. Nature 1976; 264:73-4. [More details]
Boron WF, Russell JM. Stoichiometry and ion dependencies of the intracellular-pH-regulating mechanism in squid giant axons. J Gen Physiol 1983; 81:373-99. [More details]
Boron WF. Intracellular pH transients in giant barnacle muscle fibers. Am J Physiol 1977; 233:C61-73. [More details]
Romero MF, Henry D, Nelson S, Harte PJ, Dillon AK, Sciortino CM. Cloning and characterization of a Na+-driven anion exchanger (NDAE1). A new bicarbonate transporter. J Biol Chem 2000; 275:24552-9. [More details]
Wang CZ, Yano H, Nagashima K, Seino S. The Na+-driven Cl–/HCO3- exchanger: Cloning, tissue distribution, and functional characterization. J Biol Chem 2000; 275:35486-90. [More details]
Grichtchenko II, Choi I, Zhong X, Bray-Ward P, Russell JM, Boron WF. Cloning, characterization, and chromosomal mapping of a human electroneutral Na+-driven Cl-HCO3 exchanger. J Biol Chem 2001; 276:8358-63. [More details]
Boron WF, Boulpaep EL. Intracellular pH regulation in the renal proximal tubule of the salamander: basolateral HCO3- transport. J Gen Physiol 1983; 81:53-94. [More details]
Romero MF, Hediger MA, Boulpaep EL, Boron WF. Expression cloning and characterization of a renal electrogenic Na+/HCO3- cotransporter. Nature 1997; 387:409-13. [More details]
Burnham CE, Amlal H, Wang Z, Shull GE, Soleimani M. Cloning and functional expression of a human kidney Na+:HCO3- cotransporter. J Biol Chem 1997; 272:19111-4. [More details]
Romero MF, Fong P, Berger UV, Hediger MA, Boron WF. Cloning and functional expression of rNBC, an electrogenic Na+-HCO3- cotransporter from rat kidney. Am J Physiol 1998; 274:F425-32. [More details]
Kopito RR, Lee BS, Simmons DM, Lindsey AE, Morgans CW, Schneider K. Regulation of intracellular pH by a neuronal homolog of the erythrocyte anion exchanger. Cell 1989; 59:927-37. [More details]
Deitmer JW, Schlue WR. An inwardly directed electrogenic sodium-bicarbonate cotransport in leech glial cells. J Physiol 1989; 411:179-94. [More details]
Ishiguro H, Steward MC, Lindsay AR, Case RM. Accumulation of intracellular HCO3- by Na+-HCO3- cotransport in interlobular ducts from the guinea-pig pancreas. J Physiol 1996; 495:169-78. [More details]
Zhao H, Star RA, Muallem S. Membrane localization of H+ and HCO3- transporters in the rat pancreatic duct. J Gen Physiol 1994; 104:57-85. [More details]
Bevensee MO, Apkon M, Boron WF. Intracellular pH regulation in cultured astrocytes from rat hippocampus. II. Electrogenic Na/HCO3 cotransport. J Gen Physiol 1997; 110:467-83. [More details]
Abuladze N, Lee I, Newman D, Hwang J, Boorer K, Pushkin A, Kurtz I. Molecular cloning, chromosomal localization, tissue distribution, and functional expression of the human pancreatic sodium bicarbonate cotransporter. J Biol Chem 1998; 273:17689-95. [More details]
Choi I, Romero MF, Khandoudi N, Bril A, Boron WF. Cloning and characterization of a human electrogenic Na+-HCO3- cotransporter isoform (hhNBC). Am J Physiol 1999; 276:C576-84. [More details]
Bevensee MO, Schmitt BM, Choi I, Romero MF, Boron WF. An electrogenic Na/HCO3 cotransporter (NBC) with a novel C terminus, cloned from rat brain. Am J Physiol Cell Physiol 2000; 278:C1200-11. [More details]
Marino CR, Jeanes V, Boron WF, Schmitt BM. Expression and distribution of the Na+-HCO3-cotransporter in human pancreas. Am J Physiol 1999; 277:G487-94. [More details]
Kettenmann H, Schlue WR. Intracellular pH regulation in cultured mouse oligodendrocytes. J Physiol 1988; 406:147-62. [More details]
Aalkjaer C, Hughes A. Chloride and bicarbonate transport in rat resistance arteries. J Physiol 1991; 436:57-73. [More details]
Dart C, Vaughan-Jones RD. Na+-HCO3- symport in the sheep cardiac Purkinje fibre. J Physiol 1992; 451:365-85. [More details]
Lagadic-Gossmann D, Buckler KJ, Vaughan-Jones RD. Role of bicarbonate in pH recovery from intracellular acidosis in the guinea-pig ventricular myocyte. J Physiol 1992; 458:361-84. [More details]
Choi I, Aalkjaer C, Boulpaep EL, Boron WF. An electroneutral sodium/bicarbonate cotransporter NBCn1 and associated sodium channel. Nature 2000; 405:571-5. [More details]
Pushkin A, Abuladze N, Lee I, Newman D, Hwang J, Kurtz I. Cloning, tissue distribution, genomic organization, and functional characterization of NBC3, a new member of the sodium bicarbonate cotransporter family. J Biol Chem 1999; 274:16569-75. [More details]
Pushkin A, Abuladze N, Lee I, Newman D, Hwang J, Kurtz I. Mapping of the human NBC3 (SLC4A7) gene to chromosome 3p22. Genomics 1999; 57:321-2. [More details]
Pushkin A, Abuladze N, Newman D, Kurtz I. The C-terminus of NBC3 has a PDZ domain which mediates its interaction with the vacuolar H+-ATPase. J Am Soc Nephrol 2000; 11:8A. [More details]
Hogan EM, Cohen MA, BoronWF. K+- and HCO3- dependent acid-base transport in squid giant axons: I. Base efflux. J Gen Physiol 1995; 106:821-44. [More details]
Hogan EM, Cohen MA, Boron WF. K+- and HCO3- dependent acid-base transport in squid giant axons: II. Base influx. J Gen Physiol 1995; 106:845-62. [More details]
Zhao J, Hogan EM, Bevensee MO, Boron WF. Out-of-equilibrium CO2/HCO3- solutions and their use in characterizing a new K/HCO3 cotransporter. Nature 1995; 374:636-9. [More details]
Melvin JE, Park K, Richardson L, Schultheis PJ, Shull GE. Mouse down-regulated in adenoma (DRA) is an intestinal Cl–/HCO3- exchanger and is up-regulated in colon of mice lacking the NHE3 Na+/H+ exchanger. J Biol Chem 1999; 274:22855-61. [More details]
Soleimani M. Impaired pancreatic ductal bicarbonate secretion in cystic fibrosis. JOP. J Pancreas (Online) 2001; 2(4 Suppl): 237-242. [More details]
Scott DA, Karniski LP. Human pendrin expressed in Xenopus laevis oocytes mediates chloride/formate exchange. Am J Physiol Cell Physiol 2000; 278:C207-11. [More details]
Soleimani M, Greeley T, Petrovic S, Wang Z, Amlal H, Kopp P, Burnham CE. Pendrin: an apical Cl–/OH–/HCO3- exchanger in the kidney cortex. Am J Physiol 2001; 280:F356-64. [More details]
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Key words Bicarbonates; Cloning, Organism; Hydrogen-Ion Concentration; Ion Transport; Kidney Tubules, Proximal; Oocytes; Pancreatic Ducts
Abbreviations AE: anion exchanger; BT: bicarbonate transporter; DIDS: 4,4'-diisothiocyanostilbene-4,4'-disulfonate; DRA: down-regulated in adenoma: EIPA: ethylisopropylamiloride; KBC: K/HCO3 cotransporter; NBCe: electrogenic Na/HCO3 cotransporter; NBCn: electroneutral Na/HCO3 cotransporter; NDAE: Na+-driven anion exchanger; NDCBE: Na+-driven Cl-HCO3 exchanger; pHi: intracellular pH; PPTEA: phenyl-propyltetraethylammonium; SAT: sulfate anion transporter; Vm: membrane voltage
Acknowledgements Supported by NIH grants R01-DK30344, NS18400 and R01-P01 HD32573.
Walter F Boron
Department of Cellular and Molecular Physiology
333 Cedar St
New Haven, CT 06520-8026
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